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In recent years, accumulating evidence has uncovered the complex activation pathways of pyroptosis in melanoma cells and clarified its critical role in linking cell death to inflammatory signaling and immune activation ( 3.1 Gasdermin protein-mediated execution mechanism of pyroptosis Pyroptosis is a form of regulated inflammatory cell death primarily executed by the gasdermin (GSDM) family of proteins, which includes members such as GSDMD and GSDME (71, 72)

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The rapid and continuous depletion of GSH was observed upon adding H 2 O 2 into the PtCu 3 -PEG nanocages solution

For measurement of metabolite levels in the medium, the medium was removed prior to harvesting the hepatocytes and immediately frozen and stored at 80 C for later analysis

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VSMC ferroptosis and cell senescence in vascular diseases Ferroptosis may also contribute to VSMC senescence, a process implicated in the development of atherosclerosis and vascular calcification