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Stimulation of proteoglycan production by glucosamine sulfate in chondrocytes isolated from human osteoarthritic articular cartilage in vitro

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Briefly, sitting drop crystallisations were set up in 96-well trays using a Cartesian robot with a reservoir solution of 1.5 M ammonium sulfate and 0.1 M Tris at pH 8.5, each crystallisation drop containing 100 nL each of protein and reservoir solutions